human non small cell lung cancer cell lines a549 Search Results


a549 human non-small-cell lung carcinoma cells  (Pharmaseed Ltd)
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Pharmaseed Ltd a549 human non-small-cell lung carcinoma cells
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akebia trifoliata  (Guiyang Xintian Pharmaceutical Co Ltd)
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pancreatic cancer cell line mia-paca-2  (AtaGenix Inc)
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human lung cancer cell line a549  (PeproTech)
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cell line a549 human lung cancer  (Panapharm Laboratories)
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a549 cell assays  (Evotec Inc)
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human non-small cell lung cancer cells a549  (System Biosciences Inc)
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System Biosciences Inc human non-small cell lung cancer cells a549
Human Non Small Cell Lung Cancer Cells A549, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human lung cancer cell lines a549  (BioTherapeutics Inc)
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BioTherapeutics Inc human lung cancer cell lines a549
A novel tumor-selective oncolytic VVL-m12 replicates efficiently in and is cytotoxic to lung cancer cell lines. (A) Vaccinia virus Lister strain with deletions of the thymidine kinase (TK) and N1L genes has been described previously . We retained the original B5R gene and placed the signal peptide, stalk (S), transmembrane (T) and cytoplasmic tail (C) regions (STC) in the deleted four short consensus repeat (SCR) domains under an H5 promoter control within the TK region to obtain VV CTRL . IL-12 (murine (m) or human (h)) was incorporated into the N1L region of VV CTRL under control of the H5 promoter, creating VVL-m/h12. (B) Cytotoxicity of VVLΔTKΔN1L, VV CTRL and VVL-m12 against murine lung cancer LLC, CMT64, CMT167 and <t>CMT170</t> cells and normal murine NIH3T3 cells. Cell death was determined by MTS assay 144 hours post-infection. EC50 values ± SEM are shown. One-way ANOVA with post hoc Tukey tests were used to assess significance (n=3/group). (C, D) Production of infectious virions after infection of murine lung cancer cell lines and normal murine cells over time. Virus production from whole cell lysates was assessed (C) and EEV production was determined via titration of viral supernatant from the same experiments (D) . Mean PFU/cell ± SEM is shown at each time-point and statistical significance determined using two-way ANOVA with Tukey’s multiple comparison post-test (n=3/group). (E) IL-12 expression after infection of murine lung cancer cell lines at an MOI of 1 PFU/cell. Supernatant was collected every 24 hours for 72 hours and assayed for IL-12 by ELISA. Data were normalized to cell number infected and displayed as ng/2×10 5 cells. n=3/group. In all cases, the mean ± SEM is shown. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
Human Lung Cancer Cell Lines A549, supplied by BioTherapeutics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human non-small-cell lung a549-ptx and a549-ptx-re cell lines  (Keygen Biotech)
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Keygen Biotech human non-small-cell lung a549-ptx and a549-ptx-re cell lines
A novel tumor-selective oncolytic VVL-m12 replicates efficiently in and is cytotoxic to lung cancer cell lines. (A) Vaccinia virus Lister strain with deletions of the thymidine kinase (TK) and N1L genes has been described previously . We retained the original B5R gene and placed the signal peptide, stalk (S), transmembrane (T) and cytoplasmic tail (C) regions (STC) in the deleted four short consensus repeat (SCR) domains under an H5 promoter control within the TK region to obtain VV CTRL . IL-12 (murine (m) or human (h)) was incorporated into the N1L region of VV CTRL under control of the H5 promoter, creating VVL-m/h12. (B) Cytotoxicity of VVLΔTKΔN1L, VV CTRL and VVL-m12 against murine lung cancer LLC, CMT64, CMT167 and <t>CMT170</t> cells and normal murine NIH3T3 cells. Cell death was determined by MTS assay 144 hours post-infection. EC50 values ± SEM are shown. One-way ANOVA with post hoc Tukey tests were used to assess significance (n=3/group). (C, D) Production of infectious virions after infection of murine lung cancer cell lines and normal murine cells over time. Virus production from whole cell lysates was assessed (C) and EEV production was determined via titration of viral supernatant from the same experiments (D) . Mean PFU/cell ± SEM is shown at each time-point and statistical significance determined using two-way ANOVA with Tukey’s multiple comparison post-test (n=3/group). (E) IL-12 expression after infection of murine lung cancer cell lines at an MOI of 1 PFU/cell. Supernatant was collected every 24 hours for 72 hours and assayed for IL-12 by ELISA. Data were normalized to cell number infected and displayed as ng/2×10 5 cells. n=3/group. In all cases, the mean ± SEM is shown. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
Human Non Small Cell Lung A549 Ptx And A549 Ptx Re Cell Lines, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A novel tumor-selective oncolytic VVL-m12 replicates efficiently in and is cytotoxic to lung cancer cell lines. (A) Vaccinia virus Lister strain with deletions of the thymidine kinase (TK) and N1L genes has been described previously . We retained the original B5R gene and placed the signal peptide, stalk (S), transmembrane (T) and cytoplasmic tail (C) regions (STC) in the deleted four short consensus repeat (SCR) domains under an H5 promoter control within the TK region to obtain VV CTRL . IL-12 (murine (m) or human (h)) was incorporated into the N1L region of VV CTRL under control of the H5 promoter, creating VVL-m/h12. (B) Cytotoxicity of VVLΔTKΔN1L, VV CTRL and VVL-m12 against murine lung cancer LLC, CMT64, CMT167 and CMT170 cells and normal murine NIH3T3 cells. Cell death was determined by MTS assay 144 hours post-infection. EC50 values ± SEM are shown. One-way ANOVA with post hoc Tukey tests were used to assess significance (n=3/group). (C, D) Production of infectious virions after infection of murine lung cancer cell lines and normal murine cells over time. Virus production from whole cell lysates was assessed (C) and EEV production was determined via titration of viral supernatant from the same experiments (D) . Mean PFU/cell ± SEM is shown at each time-point and statistical significance determined using two-way ANOVA with Tukey’s multiple comparison post-test (n=3/group). (E) IL-12 expression after infection of murine lung cancer cell lines at an MOI of 1 PFU/cell. Supernatant was collected every 24 hours for 72 hours and assayed for IL-12 by ELISA. Data were normalized to cell number infected and displayed as ng/2×10 5 cells. n=3/group. In all cases, the mean ± SEM is shown. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Journal: Frontiers in Immunology

Article Title: A novel oncolytic Vaccinia virus armed with IL-12 augments antitumor immune responses leading to durable regression in murine models of lung cancer

doi: 10.3389/fimmu.2024.1492464

Figure Lengend Snippet: A novel tumor-selective oncolytic VVL-m12 replicates efficiently in and is cytotoxic to lung cancer cell lines. (A) Vaccinia virus Lister strain with deletions of the thymidine kinase (TK) and N1L genes has been described previously . We retained the original B5R gene and placed the signal peptide, stalk (S), transmembrane (T) and cytoplasmic tail (C) regions (STC) in the deleted four short consensus repeat (SCR) domains under an H5 promoter control within the TK region to obtain VV CTRL . IL-12 (murine (m) or human (h)) was incorporated into the N1L region of VV CTRL under control of the H5 promoter, creating VVL-m/h12. (B) Cytotoxicity of VVLΔTKΔN1L, VV CTRL and VVL-m12 against murine lung cancer LLC, CMT64, CMT167 and CMT170 cells and normal murine NIH3T3 cells. Cell death was determined by MTS assay 144 hours post-infection. EC50 values ± SEM are shown. One-way ANOVA with post hoc Tukey tests were used to assess significance (n=3/group). (C, D) Production of infectious virions after infection of murine lung cancer cell lines and normal murine cells over time. Virus production from whole cell lysates was assessed (C) and EEV production was determined via titration of viral supernatant from the same experiments (D) . Mean PFU/cell ± SEM is shown at each time-point and statistical significance determined using two-way ANOVA with Tukey’s multiple comparison post-test (n=3/group). (E) IL-12 expression after infection of murine lung cancer cell lines at an MOI of 1 PFU/cell. Supernatant was collected every 24 hours for 72 hours and assayed for IL-12 by ELISA. Data were normalized to cell number infected and displayed as ng/2×10 5 cells. n=3/group. In all cases, the mean ± SEM is shown. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Article Snippet: Mouse CMT167, as well as CMT170 lung cancer cell line, human lung cancer cell lines H1299 and A549 and non-cancerous cell lines NIH3T3 (murine) and NHBE (human) were obtained from the Centre for Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, UK.

Techniques: Virus, Control, MTS Assay, Infection, Titration, Comparison, Expressing, Enzyme-linked Immunosorbent Assay